The Vectra® 3 is a powerful system merging automated slide-handling, multispectral imaging technology, and unique pattern-recognition-based image analysis in one automated workflow. This system accurately measures protein expressions and morphometric characteristics in distinct tissue regions of interest or on whole slides. Tissue sections or TMAs can be labeled with immunofluorescent (IF) or immunohistochemical (IHC) stains, or with conventional stains (e.g., H&E). When using IF or IHC stains, multiple proteins can be measured on a per tissue, per cell, or per cell compartment (e.g. nuclear, cytoplasmic) basis - even when signals are spectrally similar, are located in the same cellular compartment or are obscured by autofluorescence.