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Nikon Ti2-E with Crest X-Light V2 Spinning Disk


The microscope Nikon Ti2-E with Crest X-Light V2 Spinning Disk is very flexible inverted fluorescence platform suited to live cell image time-lapse microscopy either in confocal or fluorescence widefield mode. Allowing high resolution TLM  or  medium/high-throughput  phenotypic screenings.

The environmental control is achieved by an IBIDI OKOLAB UNO-T-H-CO2 TOP STAGE INCUBATOR system with CO2 mixer and temperature regulation compatible with two 8well u-Slices and two 35mm

u-Dishes and 24 and 96well plates.

Applications:


  • Live-cell imaging
  • Fluorescence microscopy
  • Spinning disk confocal
  • Medium/high-content applications

Features:


  • Metal halide 100W lamp for epifluorescence
  • LED Light engine Lumencor Spectra X for Crest Spinning Disc with dual operation mode confocal/fluorescence widefield
  • Illumination / filter set compatibility: DAPI, Hoechst, CFP, GFP, FITC, YFP, TRITC, Cy3, Texas Red, mCherry, Cy5
  • Crest X-Light V2 Spinning Disc with Single Pattern - 22 mm / 40 μm Pinhole optimized for 60x Objective.
  • Dual operation mode confocal/fluorescence widefield
  • Large FOV: 22x22mm
  • Zyla 4.2 USB3 SCMOS Camera, >80%QE, 40ffps@16bit, 53 ffps@12bit.
  • OKOLAB UNO-T-H-CO2 TOP STAGE INCUBATOR system with CO2 mixer and temperature regulation compatible with two 8well u-Slices and two 35mm u-Dishes and 24 and 96well plates

Objectives

magnif.

NA

type

specific

60 x

40 x

20 x

10 x

1.4

0.95

0.75

0.5

WD 0,13mm

WD 0.16-0.25mm

WD 1mm

WD 2mm

Lambda Oil

Lambda Air

Lambda Air

Lambda Air

 

Software:


Nikon Elements software for advanced acquisition and analysis