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Zeiss Lightsheet Z.1


The Multiview Light Sheet Fluorescence Microscope enables fluorescence imagingof large, living samples, with  low phototoxicity or bleaching and with high temporal resolution. Lightsheet Z.1 allows researchers to record the development of living organisms or large tissue samples. It acquires images of the entire sample volume at sub-cellular resolution - in a fraction of the time it takes using other techniques.

Applications:


  • Fast 3D imaging of large volumes
  • long term imaging of cells, tissues and animals
  • Embryology, development
  • tissue imaging
  • 3D cell culture

Objectives

magnif.

NA

type

specific

63 x

1.00

W Plan-Apochromat

VIS-IR

20 x

1.00

W Plan-Apochromat

UV-VIS-IR Correction collar for differences in refractive index nD from -0.002 to 0.025

 

Illumination

 

Laser

405 nm

488 nm

561 nm

638 nm

 

Filter + Lightpath settings

Beamsplitters

Camera 1

Choose here

Mirror

LP 490

LP 510

LP 560

LP 560 A

LP 56 B

auto

NONE

BP 420-470

BP 420-470

BP 505-545

BP 505-545

BP 505-530



DAPI

DAPI

GFP

GFP


One color

2 colors

2 colors

2 colors

2 colors

2 colors

 

Camera 2

Choose here

Mirror

LP 490

LP 510

LP 560

LP 560 A

LP 56 B

auto

NONE

BP 420-470

BP 420-470

BP 505-545

BP 505-545

BP 505-530



GFP

Cy3

Cy3

Alexa633


One color

2 colors

2 colors

2 colors

2 colors

2 colors

 

Cameras


  • 2x PCO Edge, scientific CMOS Cameras
  • 1920 x 1920 pixel Resolution
  • water cooled
  • pixelsize 6.5 µm

 

Analysis Workstation


  • 30 TB Raid System
  • 2x Xenon 12 core Processors
  • 98 GB RAM

 

Software:


  • Zeiss Zen Software
  • FIJI
  • Amira, 3D Renderer
  • Matlab
  • Ilastik.