Inverted Zeiss LSM 700 Laser Scanning Confocal Microscope

This Zeiss LSM 700 confocal system uses a Zeiss AXIO Observer inverted microscope stand with transmitted light illumination, a light source for fluorescence excitationing HXP 120 and laser illumination sources. It can collect transmitted light images and confocal fluorescence images. It is a basic confocal microscope, useful for standard imaging techniques.

Applications:

Co-localization of multiple proteins and multicolor imaging of fixed samples.

Time-lapse recording for following cellular events in live samples

Definite focus, focus stabilizer

Spectral imaging, unmixing, excitation fingerprinting

FRET and FRAP

Z-series

Parallel time-lapse imaging at multiple positions

Objectives
magnif.	NA	type	specific
5 x		Plan-Apochromat
10 x	0.30	Plan-Neofluar
40 x	1.30	EC Plan-Neofluar	DIC/Oil immersion
63 x	1.40	Plan-Apochromat	DIC/Oil immersion


Lasers
wavelenght	type
405	Diode
488	Diode
555	Diode
639	Diode


detectors	filter or beamsplitter
Confocal spectral detector Two PMT detectors Transmitted light detector (TPMT)	MBS 405/488/555/639

Software:

ZEN 2009 software to control the microscope, scanning, laser module, tools and the image acquisition and processing.

Computer:

LSM700 is controlled via a Fuji/Siemens workstation with 3 GB of RAM and the Windows Vista (32 bit) operating system.