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Zeiss LSM 980 with Airyscan 2 confocal laser scanning microscope

The inverted microscope is equipped with a Zeiss Airyscan 2 detector, which enables high-resolution imaging with any fluorescently labeled  sample. The multiplex mode offers up to 8 times faster and more gentle imaging compared to conventional confocal microscopy, which is ideal for long-term imaging of living cells. Focus stabilization is achieved by the Definite Focus system. 

The microscope is fully enclosed with heating and CO2 environmental control and is equipped with a motorized stage.

The Zeiss LSM 980 with Airyscan 2 belongs to the Confocal Microscopy Facility of the IZKF and is located in the University Hospital. It can be booked by all researchers of RWTH Aachen University.

For more information, visit our website at

https://www.medizin.rwth-aachen.de/cms/Medizin/Forschung/Forschungsinfrastruktur/Core-Facilities/~pqgd/Confocal-Microscopy-Facility/

or contact

Prof. Dr. Gerhard Müller-Newen

gmueller-newen@ukaachen.de
Tel.: +49 241 80 88860

Dr. Sabrina Ernst

sabernst@ukaachen.de Tel.:
+49 241 80 88838

Applications:

Confocal Microscopy can be used to generate optical slices of fluorescently labeled samples and to examine living cells with advanced fluorescence techniques

Confocal images:

• Pre-experimental consulting and hands-on training
• 2-dimensional confocal images
• Multi-channel images with up to 4 fluorophores
• Fluorescence intensity profiles
• Colocalization analysis
• 3-dimensional confocal images
• Preparation of image stacks („z-stacks“)
• Reconstruction of 3-dimensional objects)

Advanced fluorescence techniques:

• High-resolution microscopy based on the Zeiss Airyscan 2 detector
• Multiplex Mode for fast and gentle imaging, especially for live cell imaging
• AI-Sample Finder for automated sample recognition and overview images
• Definite Focus system for multi position and long term experiments
• Single-molecule RNA fluorescence in situ hybridization (smRNA-FISH)
• Spectral imaging and linear unmixing for the separation of more than 4 fluorophores
• FRAP (fluorescence recovery after photobleaching) and FLIP (fluorescence loss in photobleaching) to determine the mobility of fluorescently labelled molecules in living cells
• FRET (Förster resonance energy transfer) to detect interaction of fluorescently labeled molecules