PerkinElmer Vectra 3.0 Automated Quantitative Pathology Imaging System

The Vectra® 3 is a powerful system merging automated slide-handling, multispectral imaging technology, and unique pattern-recognition-based image analysis in one automated workflow. This system accurately measures protein expressions and morphometric characteristics in distinct tissue regions of interest or on whole slides. Tissue sections or TMAs can be labeled with immunofluorescent (IF) or immunohistochemical (IHC) stains, or with conventional stains (e.g., H&E). When using IF or IHC stains, multiple proteins can be measured on a per tissue, per cell, or per cell compartment (e.g. nuclear, cytoplasmic) basis - even when signals are spectrally similar, are located in the same cellular compartment or are obscured by autofluorescence.

Applications:

Quantification of conventional stains

Multiplex IHC/IF

Cell- and compartiment-specific quantification

Pattern recognition

Elimination of autofluorescence

Large-scale analysis

Objectives
magnif.	NA	WD	type
4 x	0.16	13 mm	Universal Plan Super Apochromat
20 x	20 x	0.6 mm	Universal Plan Super Apochromat
40 x	0.95	0.18 mm	Universal Plan Super Apochromat

CCD Camera with Liquid Cristal Tunable Filter (Nunace FX) LCTF multispectral CCD camera 1392x1040 px, pixel size is 6.45µm x 6.45µm >15fps 8bit or 12bit (selectable) Detection range: 420-720nm, scanning band width: 20nm or 40nm (selectable) Separate up to 10 markers (= Max 10 colors or fluorescent spectrum including Autofluorescence)

Microscopy:

Olympus BX51 (motorized and automated x-y stage, automated filter and objective
Fluorescent filter cubes (DAPI, FITC, Texas Red, Cy3 and Cy5)
Light source (Tungsten lamp – for brightfield, Hg lamp – for fluorescence)
Scan speed : x4:90sec per 1.5cm x 1.5cm area
Microscope Controller box
Auto Slide Loader, Robotic Arm (Only Vecta 200 Slides)
Slides hotel 4x racks of 50 slides = Capacity up to 200 slides
Automatically load a slide onto the stage of microscopy via Robotic Arm
Bar code reader

Computer:

Dell Precision T3600
Windows 7 Pro, 64-bit English
Four Core Xenon E5-1620, 3.6 GHz, 10 M Turbo Dell Precision T3600 XL
Memory 16GB
Hard Drive 500 GB OS + 1 TB for data

Software:

Vectra® software controls the system. Inform® software analyzes PerkinElmer Vectra multispectral images and uses a combination of machine-learning, pattern-recognition-based tissue segmentation and multispectral imaging for automated image analysis and extraction of signals from tissue markers. Allows for quantitative analysis of biomarker expression in tissue sections and TMAs, separation of weakly expressing and overlapping markers, cellular analysis of H&E, IHC and immunofluorescence in fixed tissues, and automatic identification of specific tissue types using trainable feature recognition algorithms. The study workflow interface supports batch processing, rapid segmentation review, and data merging. Capable of exporting spectrally unmixed signals on a per-cell basis from within selected tissue-segmentation areas.

Phenochart® software enables viewing and annotation of Brightfield and Fluorescent slides scanned by PerkinElmer’s Vectra 3 Automated Quantitative Pathology Imaging System.