HOME   EXPERTISE & PEOPLE   MICROSCOPES    GALLERY    EVENTS    PARTNERS    CONTACT    INTERNAL

Inverted Zeiss LSM 710 Laser Scanning Confocal Microscope with 34-Channel Quasar Detector

The system comes with a Zeiss AXIO Observer Z1 inverted microscope stand with transmitted light illumination, light source for fluorescence excitationing HXP 120 and laser illumination sources. It can collect transmitted light images (bright field and DIC) as well as conventional and confocal fluorescence images. The microscope is fully enclosed for heating and CO2 environmental control and is equipped with a motorized stage.

This Zeiss LSM 710 can be used for the generation of confocal images of fixed samples and the investigation of living cells.

The Zeiss LSM710 belongs to the Confocal Microscopy Facility of the IZKF and is located in the University Hospital. It can be booked by all researchers of RWTH Aachen University.

For more information, visit our website at

https://www.medizin.rwth-aachen.de/cms/Medizin/Forschung/Forschungsinfrastruktur/Core-Facilities/~pqgd/Confocal-Microscopy-Facility/

or contact

Prof. Dr. Gerhard Müller-Newen

gmueller-newen@ukaachen.de

Tel.: +49 241 80 88860

Dr. Sabrina Ernst

sabernst@ukaachen.de Tel.:

+49 241 80 88838

Applications:

Confocal images:

• Pre-experimental Consulting

• 2-dimensional confocal Images
  - Multi-channel images with up to 4 fluorophores
  - Spectral imaging, unmixing, excitation fingerprinting
  - Exact overlay of fluorescence and differential   interference constrast (DIC) Images
  - Fluorescence intensity profiles
  - Colocalization Analysis

• 3-dimensional confocal Images
  - Preparation of image stacks („z-stacks“)
  - Reconstruction of 3-dimensional objects

Advanced microscopy:

• Pre-experimental Consulting FRAP (fluorescence recovery after photobleaching and FLIP (fluorescence loss in photobleaching) to determine the mobility of fluorescently labeled molecules in living cells

• FLAP (fluorescence localization after photobleaching) to determine shuttling of fluorescently labeled molecules between subcellular compartments

• FRET (fluorescence resonance energy transfer) to detect interaction of fluorescently labeled molecules

• Use of photoconvertible and photoactivatable fluorescent proteins to determine protein dynamics in living cells

• Parallel live cell imaging at multiple positions with focus stabilization (Definite Focus)