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LaVision BioTec TriMScope I, two photon microscope

The TriM Scope II is a modular 2-photon microscope that can be adapted to various applications from developmental biology, cancer research and immunology to neuroscience. Thereby, different types of measurements from 3D time-lapse experiments to fast calcium imaging are possible.

Applications:

Laser:  Spectra Physics MaiTai HP

Laser modulation: LaVision BioTec TriMScope I with fixed pre-chirp, optical beam multiplicator and non-resonance mirror scan head

Microscope stand:

BX61WIF (motorized upright microscope)

Detection:

Single beam mode: filter based detection system. Imaging with up to six detectors is possible (three direct PMT detectors (d=25 mm) at the normal PMT port and three GaAsP detectors (Hamamatsu H 7422-40) at the ultrasensitive PMT port with optional exchange of detectors between the ports). One detector port in transmission below the sample stage (usually not occupied). Spectral filtering is performed by manual selection of dichroic mirrors and bandwidth filters in front of each PMT.

Multi-beam mode:

Two highly sensitive Hamamatsu C9100-02 EMCCD cameras with motorized filter wheel for spectral filtering.

Fluorescent filters available from 380-670 nm in approx. 20 nm and 50 nm bandwidth steps (including corresponding dichroic mirrors).

Microscope Stage:

Motorized LaVision BioTec intravital X/Y stage.

Incubation system:

Live imaging services incubation system CUBE&BOX. Sensor: Pt-100 thin-layer RTD temperature range: +5° C to 50°C.

Image acquisition software:

LaVision BioTec Imspector Pro

Others:

Classical HBO fluorescence light source (X-Cite120) including light guide, four fluorescent filter cubes, bright field trans-illumination light source (TH4-200) with an aplanatic achromat 1.4 condenser.

Trigger box for the transformation of external signals (ECG and respiration) into trigger pulses for in vivo imaging.