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Inverted Zeiss LSM 710 Multiphoton Airyscan

Laser Scanning Confocal Microscope

This Zeiss LSM 710 confocal system uses a Zeiss AXIO Observer Z1 inverted microscope stand with transmitted light illumination, a light source for fluorescence excitation using HXP 120 and laser illumination sources. It collects transmitted light images (bright field and DIC) as well as conventional and confocal fluorescence images and has superresolution capabilities (Airyscan). The microscope is fully enclosed for heating and CO2 control and is equipped with a motorized stage.

Wide array of imaging applications including protein localization analysis,  live cell imaging of cells and animals.

Applications:

• Co-localization of multiple proteins and multicolor imaging of fixed samples.

• Time-lapse recording for following cellular events in live samples

• Spectral imaging, unmixing, excitation fingerprinting

• Superresolution imagining with Airscan (1.7X improvement over diffraction-limited imaging)

• Ion Imaging

• FRET, FRAP and FLIP

• Photoactivation/ -conversion

• Z-series

• Parallel time lapse imaging at multiple positions

• Stitching

• Imaging of weak fluorescence signals